Journal: Cancers

Article Title: Correlation between High PD-L1 and EMT/Invasive Genes Expression and Reduced Recurrence-Free Survival in Blood-Circulating Tumor Cells from Patients with Non-Muscle-Invasive Bladder Cancer

doi: 10.3390/cancers13235989

Figure Lengend Snippet: Confocal microscopy representative images of isolated CTCs stained for epithelial and hematopoietic markers. Representative image by confocal microscopy of circulating cancer cells stained with anti-human EPCAM, anti-human pan-CK or anti-human CD45 Abs followed by goat anti-mouse secondary antibody Alexa 594-conjugated. DAPI was used to counteract nuclei. Magnification 100×. CK = cytokeratin, an epithelial cytoplasmic marker; DAPI = nuclear marker; EpCAM = epithelial membrane marker; CD45 = leukocyte common antigen.

Article Snippet: Staining was performed using mouse anti-human pan-Cytokeratin (C11) Ab (PanCK; 1:50, sc-8018, Santa Cruz Biotechnology, Heidelberg, Germany), anti-human CD45 Ab (1:50, #13917, Cell Signaling Technology, Danvers, MA, USA) and anti-human EpCAM (VU1D9) Ab (1:50, #2929, Cell Signaling Technology) followed by goat anti-mouse IgG H&L Ab (Alexa Fluor ® 594, 1:100, Abcam, Cambridge, UK), labeled with PureBluTM DAPI (#1351303, BioRad) according to the manufacturer’s protocol.

Techniques: Confocal Microscopy, Isolation, Staining, Marker, Membrane

Journal: eBioMedicine

Article Title: An immune score reflecting pro- and anti-tumoural balance of tumour microenvironment has major prognostic impact and predicts immunotherapy response in solid cancers

doi: 10.1016/j.ebiom.2023.104452

Figure Lengend Snippet: Resource table.

Article Snippet: Mouse monoclonal anti-PanCK , Abcam , Clone C-11.

Techniques: Amplification, Plasmid Preparation, Staining, Software